Tyrosine kinases (PTK) represent only 10% of all protein kinases, although they are the most important protein kinases. Identification of biomarker and also the study of protein expression of the cancer are studied through proteomics platforms. Most of the proteins sug-gested in the literature as clinically useful molecules are still awaiting proper validation. Main difficulties concern both complexity and heterogeneity of ovarian cancer and drawbacks of the mass spectrometry strategies. Protein Tyrosine Kinase and Phosphatase Expression Profiling in Cancer Research. This review summarizes challenges, capabilities, and promises of the mass spectrometry-based proteomics techniques in ovarian cancer management. They said that testing cancers for the presence of this protein may help clinicians identify patients who may be resistant to or relapse when treated with the therapy. Hypothesis-testing is one of the most critical aspects of the cancer research. Proteomics in Cancer Research Asian Pacific Journal of Cancer Prevention, Vol 6, 2005 115 protein purification in cancer research. Cancer proteomics is aimed at pursuing goals such as accurate diagnosis, patient stratification, and biomarker discovery, relying on the richness of information of quantitative proteome profiles. The Clinical Proteomic Technologies for Cancer and the Early Detection Research Network are efforts to identify protein signatures specific to different types of cancers. The protein arrays have in common the placement of a small amount of target material on a solid support, and they use a variety of techniques to identify enzymatic or protein-binding activity. According to American Medical Association (AMA) and Office of Cancer Clinical Proteomics Research at National Cancer Institute (NCI), the term of proteome was taken from two words; protein and genome, so prote- was taken from protein and -ome from genome. Proteomics in cancer research: Methods and application of array-based protein profiling technologies Proteomics technologies are used for early detection and diagnosis of cancers for the development of novel therapeutic agents. PTKs are involved in cell growth signaling. These methods represent a heterogeneous group of techniques that can assess protein–protein interactions, protein modifications and tissue profiling. The absence of a protein that works to prevent tumor formation may explain why some patients are resistant to a common cancer therapy, according to Penn State Cancer Institute and Penn State College of Medicine researchers. An-other challenge in biomarker discovery is standardization Proteomics describes the global analysis of proteome, which can be defined has as the protein content of a cell, a tissue or an entire organism in a defined state. In order to understand proteomics, understanding what is proteome is necessary. 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